Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri ...

Biology educators emphasize aseptic technique when teaching lab courses. Aseptic technique is very important in microbiology to ensure safety and prevent cross-contamination. Although it can be assessed throughout the semester by examining the students' technique and observation of...

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Dec 04, 2009 · LAB 1B: Aseptic Techniques and Transfer of Microorgs. It is important to use aseptic techniques because they keep bacterial contamination to minimal levels. Note the diagrams of aseptic technique discussed in the lab manual. The procedure is quite simple, but is fundamental to other labs in the course. Practice makes perfect.

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Aseptic Technique APPENDIX 4D Tomasz Bykowski 1 and Brian Stevenson2 1Center for Medical Education, Warsaw, Poland 2Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky ABSTRACT This chapter describes common laboratory procedures that can reduce the risk of culture con-

3. Microbiology research methods. 4. Simple and complex staining techniques. Because microbial cytoplasm is usually transparent, it is necessary to stain microorganisms before they can be viewed with the light microscope.Technique Quizzes*/Pre-labs / & Assignments 80-130 Special Project** (mandatory project) 70 *Technique quizzes include demonstration of: 1) microscope usage/function, streak plate technique, aseptic technique, Gram stain technique, and 2) use of scientific method.

How To Streak For Isolated Colonies (SFIC): Mixed cultures (more than one species) can be isolated using the streak plate technique. The goal is to acquire a pure culture of one species of bacteria, in a single colony, from a mixed culture We do this by separating the microbes on the surface of agar with quadrant streaking, this method DILUTES the bacteria.

1.2 An understanding of microbiology and microbiological techniques is essential. Knowledge in the following areas is recommended: microorganisms, antimicrobial products (disinfectants, sporicides, and decontamination agents), the chemistry of disinfection, mechanism of activity of disinfectants on cells, application procedures, cleanroom ...

• Using aseptic technique, remove the applicator from its package. • Holding the applicator downward, gently squeeze the wings to release the solution. • Scrub with a back & forth motion using friction for 30 seconds on dry skin or 2 minutes on wet skin. • Do not wipe the site after cleansing the skin with chlorhexidine.

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